A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG

María-Natalia Lisa (1, 2) , Adrià Sogues (2) , Nathalie Barilone (2) , Meike Baumgart (3) , Magdalena Gil (4) , Martín Graña (4) , Rosario Durán (4) , Ricardo Biondi (5) , Marco Bellinzoni (2) , Michael Bott (5) , Pedro M. Alzari (2)

1 IBR - Instituto de Biología Molecular y Celular de Rosario [Rosario]
2 Microb. Struc. (UMR_3528 / U-Pasteur_5) - Microbiologie structurale - Structural Microbiology
3 IBG - Institute of Bio- and Geosciences [Jülich]
4 Institut Pasteur de Montevideo
5 Universitätsklinikum Frankfurt

Bacteria control the metabolic processes by which they obtain nutrients and energy in order to adapt to the environment. Actinobacteria , one of the largest bacterial phyla of major importance for biotechnology, medicine, and agriculture, developed a unique control process that revolves around a key protein, the protein kinase PknG. Here, we use genetic, biochemical, and structural approaches to study PknG in a system that regulates glutamate production in Corynebacterium glutamicum , a species used for the industrial production of amino acids.

Keywords: bacterial signaling; Corynebacterium glutamicum; protein kinases; protein structure-function

Domains: Structural Biology [q-bio.BM]; Bacteriology

María-Natalia Lisa, Adrià Sogues, Nathalie Barilone, Meike Baumgart, Magdalena Gil, et al.. A Tetratricopeptide Repeat Scaffold Couples Signal Detection to OdhI Phosphorylation in Metabolic Control by the Protein Kinase PknG. mBio, 2021, 12 (5), pp.e0171721. ⟨10.1128/mBio.01717-21⟩. ⟨pasteur-04131313⟩