CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.

Mayeul Collot (1) , Christian D Wilms (2) , Asma Bentkhayet (1) , Païkan Marcaggi (3) , Kiri Couchman (4, 5) , Serge Charpak (5, 4) , Stéphane Dieudonné (3) , Michael Häusser (2) , Anne Feltz (3) , Jean-Maurice Mallet (1)

1 UPMC - Université Pierre et Marie Curie - Paris 6
2 WIBR - Wolfson Institute for Biomedical Research
3 IBENS - Institut de biologie de l'ENS Paris
4 U1128 - Laboratoire de Neurophysiologie et Nouvelles Microscopies
5 INSERM - Institut National de la Santé et de la Recherche Médicale

The great demand for long-wavelength and high signal-to-noise Ca(2+) indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.

Domaines: Chimie organique

Mayeul Collot, Christian D Wilms, Asma Bentkhayet, Païkan Marcaggi, Kiri Couchman, et al.. CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.. eLife, 2015, 4, pp.e05808. ⟨10.7554/eLife.05808⟩. ⟨hal-01138078⟩