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HP1γ binding pre‐mRNA intronic repeats modulates RNA splicing decisions

Abstract : HP1 proteins are best known as markers of heterochromatin and gene silencing. Yet, they are also RNA-binding proteins and the HP1c/CBX3 family member is present on transcribed genes together with RNA polymerase II, where it regulates cotranscriptional processes such as alternative splicing. To gain insight in the role of the RNA-binding activity of HP1c in transcriptionally active chromatin, we have captured and analysed RNAs associated with this protein. We find that HP1c is specifically targeted to hexameric RNA motifs and coincidentally transposable elements of the SINE family. As these elements are abundant in introns, while essentially absent from exons, the HP1c RNA association tethers unspliced pre-mRNA to chromatin via the intronic regions and limits the usage of intronic cryptic splice sites. Thus, our data unveil novel determinants in the relationship between chromatin and co-transcriptional splicing.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03303092
Contributor : Hugo Varet Connect in order to contact the contributor
Submitted on : Wednesday, July 28, 2021 - 8:08:27 AM
Last modification on : Tuesday, November 16, 2021 - 4:29:47 AM
Long-term archiving on: : Friday, October 29, 2021 - 6:10:58 PM

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Christophe Rachez, Rachel Legendre, Mickaël Costallat, Hugo Varet, Jia Yi, et al.. HP1γ binding pre‐mRNA intronic repeats modulates RNA splicing decisions. EMBO Reports, EMBO Press, 2021, pp.e52320. ⟨10.15252/embr.202052320⟩. ⟨pasteur-03303092⟩

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